Studies on Secondary Metabolites of Streptomyces gossypiisoli TRM 44567 under the Guidance of OSMAC Strategy

作者：Y. Chen, CHANG LI, Li Xing, MIAO XU, YANG LIU, Min Ren, Xiaoxia Luo · 发表于：Polish Journal of Microbiology · 年份：2025 · DOI：10.33073/pjm-2025-001 · 研究领域：Microbial Natural Products and Biosynthesis、Microbial Metabolism and Applications、Plant biochemistry and biosynthesis

Abstract Streptomycetes synthesize a wide variety of biologically active compounds, such as antimicrobials, enzyme blockers, insecticides, and weed killers, offering the potential for use in farming as agents for enhancing plant health and defense. Streptomyces gossypiisoli TRM44567 was a novel strain isolated from continuously cropped cotton fields. An analysis of bioinformatics revealed that there are numerous natural product biosynthesis gene clusters with unknown functions in the genome sequence. Thirty-five potential natural product biosynthetic gene clusters were discovered from the genome of S. gossypiisoli TRM44567 by antiSMASH analysis. Furthermore, it was found that the strain TRM 44567 genome contained a cluster of tirandamycin biosynthetic gene cluster. Using the OSMAC strategy, screening revealed R5 medium was the most suitable medium. Based on modern isolation techniques, the fermentation product of strain TRM 44567 was identified as tirandamycin A and found to have inhibitory activity against Pseudomonas aeruginosa . The current research examines the genotypic traits of S. gossypiisoli TRM44567, suggesting its potential as a valuable reservoir of advantageous secondary compounds for medicinal and biotechnological uses.