Visceral Leishmaniasis Unresponsive to Pentostam caused by Leishmania Tropica in Kenya

作者：Yemane B. Mebrahtu, Phillip G. Lawyer, John I. Githure, J.B.O. Were, Richard Muigai, Larry D. Hendricks, Johannis Leeuwenburg, Davy Koech, Clifford R. Roberts · 发表于：American Journal of Tropical Medicine and Hygiene · 年份：1989 · DOI：10.4269/ajtmh.1989.41.289 · 被引用次数：73 · 研究领域：Research on Leishmaniasis Studies、Trypanosoma species research and implications、Biochemical and Molecular Research

We report the characterization of 6 Leishmania tropica isolates from 2 patients with visceral leishmaniasis who were unresponsive to treatment with sodium stibogluconate. The Leishmania isolates, MHOM/KE/81/NLB-029A, -029XIB, and -029XIC and MHOM/KE/81/NLB-030I, -030B, and -030XXA, all from splenic aspirates, were characterized by cellulose acetate electrophoresis using 11 enzymes: malate dehydrogenase, malic enzyme, phosphogluconate dehydrogenase, glucose-6-phosphate dehydrogenase, superoxide dismutase, glutamate-oxaloacetate transaminase, adenylate kinase, nucleoside hydrolase, mannose phosphate isomerase, glucose phosphate isomerase, and phosphoglucomutase. Isozyme migration patterns were indistinguishable from those of 2 WHO reference strains of Leishmania tropica (MHOM/SU/60/LRC-L39, NLB-305 and MHOM/IQ/OO/LRC-L36, NLB-067). These are the first reported cases of visceral leishmaniasis (kala-azar) caused by L. tropica in Africa; these cases were refractory to sodium stibogluconate.