AVIDIN. 4. STABILITY AT EXTREMES OF PH AND DISSOCIATION INTO SUB-UNITS BY GUANIDINE HYDROCHLORIDE

作者：NM GREEN · 发表于：Biochemical Journal · 年份：1963 · DOI：10.1042/bj0890609 · 被引用次数：104 · 研究领域：Biotin and Related Studies、Pesticide and Herbicide Environmental Studies、Photosynthetic Processes and Mechanisms

The great stability of avidin over a wide pH range, particularly when combined with biotin, has frequently been noted and has been studied qualitatively by Fraenkel-Conrat, Snell & Ducay (1952b).In the present paper a more detailed investigation was made by the technique of dif-ference spectrophotometry.The behaviour of avidin in concentrated solutions of urea and guanidine was also examined since it appeared likely that the molecule was built up from sub-units (Melamed & Green, 1963).The great stability, coupled with a molecular weight which was unchanged by 8M- urea (Fraenkel-Conrat, Snell & Ducay, 1952a), suggested that the sub-units might be linked covalently.Therefore attempts were made to separate