Quantification of Protein Complexes by Blue Native Electrophoresis

作者：Juliana Heidler, Valentina Strecker, Florian Csintalan, Lea Bleier, Ilka Wittig · 发表于：Methods in molecular biology · 年份：2013 · DOI：10.1007/978-1-62703-487-6_23 · 被引用次数：9 · 研究领域：Mitochondrial Function and Pathology、ATP Synthase and ATPases Research、Metabolism and Genetic Disorders

Blue native electrophoresis (BNE) is a long established method for the analysis of native protein complexes. Applications of BNE range from investigating subunit composition, stoichiometry, and assembly of single protein complexes to profiling of whole complexomes. BNE is an indispensible tool to diagnostically analyze cells and tissues from patients with mitochondrial disorders or model organisms. Since functional proteomic studies often require quantification of protein complexes, we describe here different quantification methods subsequent to protein complex separation by BNE.