Endothelial Dysfunction Contributes to Chondrocyte Senescence Associated With Insulin‐Like Growth Factor‐Binding Protein‐6 in a Spontaneously Hypertensive Rat Model

作者：Yuqi Zhang, Karen Zhi Ching, Lanlan Zhang, Lin Zhu, Siu Ling Yip, Xin Lü, Zhongyu Liu, Zongwei Cai, Man Ting Au, Chunyi Wen · 发表于：Journal of the American Heart Association · 年份：2025 · DOI：10.1161/jaha.124.042132 · 被引用次数：2 · 研究领域：Growth Hormone and Insulin-like Growth Factors、Osteoarthritis Treatment and Mechanisms、TGF-β signaling in diseases

BACKGROUND: Given an epidemiological association between hypertension and osteoarthritis, we aim to elucidate how vascular pathology triggers avascular articular cartilage loss by comparing vascular and joint phenotypes between spontaneously hypertensive rats and normotensive Wistar Kyoto rats at tissue, cellular, and molecular levels. METHODS: Systemic and local endothelial function were assessed via blood pressure monitoring and photoacoustic imaging of knee joints, respectively (n=5-6). Tissue and cellular damages in articular cartilage, subchondral bone, and synovium were systemically evaluated by radiological and histological examination (n=6). Endothelial transcriptional changes were delineated via bulk RNA-seq (n=3). The secretome of human endothelial cells under oxidative stress was analyzed via proteomics to identify key factors in chondrocyte senescence (n=4). Captopril, an antihypertensive medication, was adopted as a tool to rescue chondrocyte senescence and osteoarthritis both in vitro (n=3) and in vivo (n=6). RESULTS: Compared with Wistar Kyoto rats, spontaneously hypertensive rats exhibited higher blood pressure and local joint hypoxia with increased endothelial oxidative stress as early as 3 months old. Notably, these endothelial dysfunctions preceded the onset of structural joint damage. By 9 months of age, spontaneously hypertensive rats developed osteoarthritis-like pathology characterized by senescent chondrocyte accumulation and cartilage degradation, whi...