Nitrogen addition accelerated straw in-situ decomposition by promoting specific microbial taxa growth and straw decomposing enzyme activities

作者：Tengfei Guo, Mengyuan Wang, Y. Chen, Ke Yue, Long Ma, Shaomin Huang, Xinpeng Xu, Xiao Song, Sumiao Su, Zekun Zhang, Qian Zhang, Keke Zhang · 发表于：Frontiers in Plant Science · 年份：2025 · DOI：10.3389/fpls.2025.1703916 · 被引用次数：9 · 研究领域：Enzyme-mediated dye degradation、Mycorrhizal Fungi and Plant Interactions、Biofuel production and bioconversion

Introduction Crop residue represents the largest input of organic carbon in agricultural ecosystems and its decomposition is fundamentally mediated by soil microbial communities. However, the mechanism of N fertilization regulating decomposition of the plant residue especially the associated key microbial taxa remain unclear. Methods To address this gap, we conducted a 100-day field decomposition experiment using the litterbag method to track temporal shifts in straw physicochemical properties and associated microbial communities under three N regimes: no nitrogen (N0), 200 kg N ha -1 (N200), and 300 kg N ha -1 (N300). Results and discussions Results showed that nitrogen addition significantly accelerated the decomposition of wheat straw, increasing mass loss and the degradation rates of cellulose, hemicellulose, and lignin relative to N0 treatment. Enzyme activities linked to carbon acquisition, including α-glucosidase (AG), β-glucosidase (BG), cellobiohyrolase (CBH), and β-xylosidase (XYL), were consistently elevated under N-amended treatments during mid- to late-stage decomposition. Similarly, activities of N-acquiring enzymes (β-N-acetyl-glucosaminidase, NAG; leucine aminopeptidase, LAP) and oxidative enzymes (polyphenol oxidase, PPO; laccase) were significantly enhanced, particularly after Day 14. Microbial community succession was tightly coupled with decomposition progression. Random forest modeling identified key bacterial biomarkers (e.g., Terribacillus , Bacillus , ...