Food-residue-level antibiotics promote mucosal colonization of foodborne antibiotic-resistant Staphylococcus aureus in a simulated human gut

作者：Zehua Yan, Xiaohua Zhang, Tim Fat Shum, Jiawen Xie, Jiachi Chiou, Jun Yu, Xiangdong Li · 发表于：Gut Microbes · 年份：2025 · DOI：10.1080/19490976.2025.2599517 · 被引用次数：2 · 研究领域：Bacterial biofilms and quorum sensing、Pharmaceutical and Antibiotic Environmental Impacts、Microbial Community Ecology and Physiology

Antibiotic-resistant bacteria (ARB) and food-residue-level antibiotics in food can disrupt gut homeostasis. However, the impact of co-exposure with food-residue-level antibiotics on compartment-specific colonization dynamics and associated risks of ARB in human gut remains unclear. Here, we isolated a ciprofloxacin (CIP)-resistant Staphylococcus aureus strain from edible fish parts in aquaculture environment and assessed exposure risks to luminal and mucosal microbiotas using the in vitro Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME; ProDigest, Belgium) under three treatments: S. aureus alone, food-residue-level CIP alone, and co-exposure to both. Food-residue-level CIP promoted the potential colonization of S. aureus and relative abundance of antibiotic resistance gene hosts in the mucosal microbiota and decreased absolute abundance of 16S rRNA genes in luminal microbiota. Accordingly, microbiota exhibited compartment-specific responses: luminal microbiota exhibited increased stress tolerance potential and a tightly connected network with fewer nodes, whereas mucosal microbiota displayed enhanced resource utilization potential and a more complex network with more nodes. To investigate the mechanisms underlying these compartment-specific responses, we analyzed the microbial interconnections and enriched functions in luminal and mucosal microbiota. Notably, mucosal microbiota showed stronger positive cohesions (i.e., abundance-weighted positive correl...