Isolation and Probiotic Functions of Bacillus subtilis and Its Inhibitory Effects on Colitis

作者：Ningning Guan, Chang Li, Wei Liu, Qiong Wu, Jiajia Zhu, Ting Gao, Hui Song, Rui Guo, Fangyan Yuan, Yongxiang Tian, Keli Yang, Danna Zhou · 发表于：Biology · 年份：2025 · DOI：10.3390/biology14121786 · 被引用次数：2 · 研究领域：Probiotics and Fermented Foods、Gut microbiota and health、Animal Nutrition and Physiology

Bacillus subtilis, as a probiotic feed additive, has been increasingly applied in livestock and poultry farming. In the present study, the environmental tolerance of a strain of Bacillus subtilis, isolated from a goat farm, was investigated. This article conducts a series of experiments on the obtained strains. The results demonstrated that the isolated strain exhibits strong tolerance to high temperatures, acidic and alkaline conditions, and high concentrations of bile salts. Furthermore, its self-aggregation rate exceeded 60% after 24 h. Whole-genome sequencing revealed that the genomes of the isolated strains were functionally annotated, identifying genes associated with amino acid metabolism, vitamin biosynthesis, and other metabolic pathways. Based on this genomic analysis, the present study further evaluated the anti-inflammatory effects of a Bacillus subtilis strain isolated in a murine model of colitis induced by dextran sulfate sodium (DSS). The analyses revealed that the DSS-treated group exhibited significantly reduced expression of intestinal tight junction proteins ZO-1 and Occludin, along with elevated expression of pro-inflammatory cytokines, compared to the PBS control group. Following oral administration of 1 × 108 CFU/mL Bacillus subtilis isolated strain suspension, the DSS-treated mice showed increased expression of ZO-1 and Occludin and decreased levels of pro-inflammatory cytokines. These results indicate that the isolated strain of Bacillus subtilis has ...