A GmeIF2B5 ‐ GmPRX4 Regulatory Axis Divergently Governs Drought‐Lignin and Salt‐Ion Homeostasis in Soybean

作者：Juan Liu, Yanzhong Huang, Xiaowan Fang, Han Gou, Huidong Xuan, Sushuang Deng, Lu Li, Yanjia Wang, Xiushuai Wang, Ling Gan, Nannan Zhang, Haoran Luo, Yaolan Bai, Qing Liu, Han Xing, Jinming Zhao, Na Guo · 发表于：Plant Biotechnology Journal · 年份：2025 · DOI：10.1111/pbi.70507 · 被引用次数：2 · 研究领域：Plant Gene Expression Analysis、Plant Stress Responses and Tolerance、Photosynthetic Processes and Mechanisms

ABSTRACT Abiotic stresses severely constrain soybean productivity worldwide. Here we demonstrate that gmeif2b5 (eukaryotic initiation factors) mutants confer dual stress tolerance through coordinated mechanisms. Integrative RNA‐Seq and protein interaction analyses revealed that gmeif2b5 mutants increase lignin deposition to increase drought resilience and balanced Na + /K + homeostasis to enhance salt tolerance; GmeIF2B5 physically interacts with GmPRX4, a plant heme peroxidase; GmPRX4 overexpression increases drought and salt resistance in soybean; GmeIF2B5 plays the predominant role in the GmeIF2B5‐GmPRX4 module, and double mutants exhibiting synergistic stress tolerance improvements. Our work uncovers a ‘GmeIF2B5‐GmPRX4 regulatory axis’ that: mobilises lignin‐based structural fortification for enhanced drought resistance and orchestrates ionic equilibrium for increased salt tolerance. This study pioneers the role of eIF2B genes in soybean stress adaptation, establishing a multi‐tiered regulatory node for precision molecular design of stress‐resilient crops.