Optimized fertilizer–microbe ratios enhance synergistic restoration of alpine mining ecosystems

作者：Zongcheng Cai, Jianjun Shi, Shouquan Fu, Fayi Li, Liangyu Lv, Qingqing Liu, Hairong Zhang, Shancun Bao · 发表于：Frontiers in Microbiology · 年份：2025 · DOI：10.3389/fmicb.2025.1709528 · 被引用次数：6 · 研究领域：Microbial Community Ecology and Physiology、Soil Carbon and Nitrogen Dynamics、Mining and Resource Management

Introduction To address the critical issues of soil fertility depletion, poor vegetation establishment, and functional degradation of microbial communities in degraded alpine mining ecosystems, this study focuses on the Muli coal mine on the Qinghai–Tibet Plateau. This study systematically examined the synergistic regulatory mechanisms underlying the combined application of compound microbial inoculants and forage-specific fertilizers for restoring degraded ecosystems. The findings establish a theoretical framework and technical approach for rehabilitating fragile alpine ecosystems under extreme environmental conditions. Methods A three-year field experiment was conducted using a long-term plot design. Integrated assessments of vegetation performance, soil physicochemical properties, and soil bacterial community structure (via high-throughput 16S rRNA gene sequencing) were performed. Multivariate statistical analyses, including redundancy analysis (RDA) and structural equation modeling (SEM), were employed to evaluate the comprehensive effects of fertilizer–microbe co-application on the vegetation–soil–microorganism system. Results and discussion The optimized fertilizer–microbe treatment (W3J1: 375.00 kg·hm −2 forage-specific fertilizer + 350.00 kg·hm −2 compound microbial inoculant) significantly promoted vegetation growth, increased soil carbon and nitrogen contents, and effectively alleviated soil salinization. This treatment reshaped soil bacterial community structure, e...