Gut Phage Biobank: a collection of bacteriophages targeting human commensal bacteria

作者：Bo Xing, Cong Liu, Wantong Chen, Zhuoran Li, Xiaohuan Jing, Chao‐Liang Wu, Ruixin Liu, Xiuqin Zhang, Xun Xu, Junhua Li, Minfeng Xiao · 发表于：Nature Communications · 年份：2025 · DOI：10.1038/s41467-025-61946-0 · 被引用次数：6 · 研究领域：Bacteriophages and microbial interactions、Fecal contamination and water quality、Genomics and Phylogenetic Studies

The scarcity of cultivated gut bacteriophages hinders gut microbial research and application. Here we report the establishment and characterization of a Gut Phage Biobank (GPB) ( https://db.cngb.org/genomics/datasets/GDS0000055 ) through a systematic isolation workflow and containing 104 isolates that target abundant or disease-associated gut bacteria. Genomic analysis reveals high diversity among these phages, and key genes of phage-bacteria interactions. The infection matrix demonstrates high host-specificity and varying infectivity of these phages under different conditions, unveiling phage-bacteria interaction mechanisms. In-depth characterization of the phages targeting obligate anaerobes uncovers a previously undescribed family and four previously undescribed genera, one of which is more prevalent than the well-known crAss-like phages globally except in Eurafrica. Cohort analysis reveals a higher prevalence of Mediterraneibacter and Dorea and a lower prevalence of Mediterraneibacter phages in Asian disease population. In vitro and in vivo evidence of phage inhibiting Dorea highlight the potential of phages in disease intervention. This biobank represents a valuable resource for advancing gut microbial research and holds promise for manipulating microbiomes. Here, the authors present Gut Phage Biobank (GPB), containing 104 isolates that target abundant or disease-associated gut bacteria, revealing extensive viral diversity, and provide in vivo evidence for their effects ...