CD8+ T cells in the tumor microenvironment modulate the response to endocrine therapy in breast cancer

作者：Fabiana Napolitano, Yunguan Wang, Dhivya R. Sudhan, Paula I. González-Ericsson, Luigi Formisano, Nisha Unni, Shahbano Shakeel, James Zhu, Khushi Ahuja, Lei Guo, María del Rosario Chica-Parrado, Yuki Matsunaga, Pamela Luna, Chang-Ching Lin, Yasuaki Uemoto, Kyung‐min Lee, Hongli Ma, Nathaniel Evans, Alberto Servetto, Saurabh Mendiratta, Spencer Barnes, Roberto Bianco, Yisheng Fang, Lin Xu, Jeon Lee, Tao Wang, Justin M. Balko, Gordon B. Mills, Marilyne Labrie, Ariella B. Hanker, Carlos L. Arteaga · 发表于：Journal of Clinical Investigation · 年份：2025 · DOI：10.1172/jci188458 · 被引用次数：5 · 研究领域：Cancer Immunotherapy and Biomarkers、Estrogen and related hormone effects、Chemokine receptors and signaling

The role of the tumor immune microenvironment (TIME) in modulating responses to antiestrogen therapy in hormone receptor-positive (HR+) breast cancers remains unclear. We analyzed pre- and on-treatment biopsies from patients with HR+ breast cancer treated with letrozole to induce estrogen deprivation (ED). Stromal tumor-infiltrating lymphocytes, assessed by H&E staining, and immune-related gene sets, including IFN-γ signaling genes, measured by RNA-Seq, were increased in ED-resistant tumors. Cyclic immunofluorescence and spatial transcriptomics revealed an abundance of CD8+ T cells and enhanced antigen processing and immune gene signatures in ED-resistant tumors. In this group, the expression of CXCL9, CXCL10, and CXCL11 - chemokine genes involved in CD8+ T cell recruitment - and the CXCR3 receptor were upregulated both before and after letrozole treatment. CXCL11 levels were higher in conditioned media from HR+ breast cancer cells cocultured with CD8+ T cells. Both recombinant CXCL11 and coculture with CD8+ T cells promoted MCF7 and T47D cell growth in estrogen-free conditions. Finally, deletion combined with silencing of the CXCL11 receptors CXCR3 and CXCR7 in MCF7 cells impaired proliferation in response to exogenous CXCL11 and to coculture with CD8+ T cells in estrogen-free conditions. These findings suggest that CD8+ T cell-associated CXCL11 in the TIME modulated the response of HR+ breast cancer cells to estrogen suppression.