Prevalence, bacterial load, and genomic characterization of Vibrio parahaemolyticus in freshwater-farmed Litopenaeus vannamei from China

作者：Feifei Shan, Weiwei Li, Zun-hua Chu, Peibin Hou, Nian X. Sun, Ronghua Li, Xuejie Liu, Weiwei Chen, Ling Zhong, Shaofei Yan, Shenghui Cui, Yunchang Guo · 发表于：Frontiers in Microbiology · 年份：2025 · DOI：10.3389/fmicb.2025.1713237 · 被引用次数：3 · 研究领域：Invertebrate Immune Response Mechanisms、Vibrio bacteria research studies、Aquaculture disease management and microbiota

Shrimp was the leading food vehicle for Vibrio parahaemolyticus outbreaks in China, yet studies on V. parahaemolyticus in globally predominant farmed shrimp ( Litopenaeus vannamei ) - particularly in freshwater culture remain limited. This study aimed to investigate and evaluate the contamination of V. parahaemolyticus in freshwater-farmed marine shrimp ( L. vannamei ) in China. The prevalence and bacterial load of V. parahaemolyticus in shrimps were assessed by China China National Standard GB 4789.7-2013 and isolates were obtained for genomic analysis. The prevalence of V. parahaemolyticus in shrimp samples was 39.1% and the bacteria load ranged from 3.6 MPN/g to 24,000 MPN/g. Analysis on isolates demonstrated that 75.8% (94/124) were assigned to 28 sequence types (STs), whereas 24.2% STs were unknown. All isolates harbored 24 antibiotic resistance genes (ARGs), of which tet(34) , tet(35) , and bla CARB were harbored in all isolates. There was a significant correlation between bla CARB types and STs, with isolates sharing identical STs carried the same blaCARB variant. The trh + isolates (9.7%, 12/124) was simultaneously coexisted with hlyB and hlyC . All four ST79 strains harbored trh / hlyB / hlyC and bla CARB_46 . Our findings elucidated the contamination of V. parahaemolyticus in freshwater-farmed L. vannamei , with heavy bacteria load in Fujian province. The emergence of specific ST co-harboring critical virulence genes and ARGs indicating the necessity for targeted su...