Asparagus bean VunMED2 gene enhances Arabidopsis multi-stress resistance and activates VunSRC2 transcription

作者：Le Liang, Xueping Song, Wen Tang, Barkat Ali, Jing Zhang, Jianming Xie, Huanxiu Li · 发表于：Plant Physiology and Biochemistry · 年份：2025 · DOI：10.1016/j.plaphy.2025.110860 · 被引用次数：1 · 研究领域：Plant Gene Expression Analysis、Plant Stress Responses and Tolerance、Genomics, phytochemicals, and oxidative stress

stress, OE-VunMED2 plants displayed reduced oxidative damage, significantly higher antioxidant enzymes (SOD/POD/CAT) activity, and improved membrane stability compared to WT, confirming that VunMED2 enhances oxidative stress tolerance by activating antioxidant defense systems. Similarly, VunMED2 overexpression significantly reduced Nacl stress-induced reactive oxygen species accumulation, elevated antioxidant enzyme activity, and mitigated membrane damage, further demonstrating its role in Nacl stress alleviation via enhanced antioxidant mechanisms. Yeast one-hybrid and dual-luciferase reporter assays confirmed that VunMED2 activates the promoter of the downstream target gene VunSRC2. Under various stresses, the expression of VunSRC2 orthologous gene AtSRC2 was significantly up-regulated in VunMED2 overexpressing Arabidopsis, indicating that AtSRC2 is a key effector that mediates multi-stress tolerance. Therefore, VunMED2 enhances multi-stress tolerance by activating VunSRC2 and anti-oxidation, which provides a new target for stress-resistant breeding of asparagus bean.