Transcriptome analysis reveals plant hormone signal transduction mechanisms underlying freezing stress responses in Cycas panzhihuaensis

作者：Jiao Yu, Yihang Zhou, Chengzhi Xu, Fang Wang, Yanling Zheng · 发表于：BMC Plant Biology · 年份：2025 · DOI：10.1186/s12870-025-07907-7 · 被引用次数：2 · 研究领域：Botany and Geology in Latin America and Caribbean、Environmental and Cultural Studies in Latin America and Beyond、Cocoa and Sweet Potato Agronomy

BACKGROUND: Global warming intensifies climate extremes, with rising temperatures and more frequent heatwaves. This situation may make it more difficult for species in high-temperature regions, such as the Cycas panzhihuaensis. Introduction and domestication are vital for conservation, but low temperatures limit the spread of tropical and subtropical plants to higher latitudes and altitudes. Phytohormones mediate cold adaptation through complex signaling networks that regulate physiological and molecular responses. However, the hormonal regulation and molecular mechanisms underlying freezing tolerance in C. panzhihuaensis remain poorly understood. RESULTS: This study used C. panzhihuaensis as the research subject. We exposed the plant to freezing stress and measures leaf hormone levels under CK (control group), F1(-5 °C for 1.5 h), and F2(-5 °C for 6 h). Additionally, we conducted transcriptomic analysis to explore gene expression differences in plant hormone signal transduction pathways. The findings indicated that with prolonged freezing treatment, the contents of cis-12-oxophytodienoic acid (cis-OPDA), gibberellin A4 (GA4), and salicylic acid (SA) initially increased significantly but subsequently decreased markedly. After F2 treatment, abscisic acid (ABA) levels significantly decreased, whereas castasterone (CS), 1-aminocyclopropanecarboxylic acid (ACC), and cytokinin (CTK) exhibited notable increases. Through transcriptomic analysis, a total of 4,036 differentially expre...