Simultaneous inhibition of Cd and As absorption and transport in rice via coordinated cell wall sequestration, transporter regulation, and chelating ligand synthesis

作者：Lin Fu, Jiawei Deng, Changbo Zhang, Weijie Xue, Yun Deng, Xin Luo, Shuangyue Liu, Kexin Chen, Dayliana Ruiz LA O, Gilles Mailhot, Davide Vione, Marcello Brigante, Yuyao Liu · 发表于：Ecotoxicology and Environmental Safety · 年份：2025 · DOI：10.1016/j.ecoenv.2025.119528 · 被引用次数：2 · 研究领域：Plant Stress Responses and Tolerance、Aluminum toxicity and tolerance in plants and animals、Arsenic contamination and mitigation

[Glu][Cl], Cd and As content in roots decreased by 46.0 %-47.3 % and 53.8 %-56.5 %, respectively, and in the shoots by 37.5 %-38.7 % and 39.5 %-42.8 %, respectively. [Glu][Cl] significantly increased the proportion of Cd and As in the rice cell wall. Additionally, [Glu][Cl] downregulated OsNramp5 expression and upregulated OsGLR3 expression, which is associated with the transmembrane transport of Cd. OsLsi1,2 expression was down-regulated, which mediate the entry of As into cells and xylem. Quantum chemical calculations indicated that Cd/As can be chelated by glutamate, Cysteine, glutathione, and other substances, whose synthesis was promoted by the addition of [Glu][Cl]. In conclusion, [Glu][Cl] inhibited Cd/As absorption and transport in rice by enhancing cell wall sequestration, regulating relevant gene expression, and promoting the synthesis of chelating ligands, which provides new strategies for safe rice production in heavy metal-contaminated environments.