Nucleosome unwrapping and PARP1 allostery drive affinities for chromatin and DNA breaks

作者：Matthew A. Schaich, Tyler Weaver, Jennifer A Rakowski, Vera Roginskaya, Liam P. Leary, Aafke A. van den Berg, Janet Iwasa, Bret Freudenthal, Bennett Van Houten · 发表于：Nature Communications · 年份：2025 · DOI：10.1038/s41467-025-67071-2 · 被引用次数：4 · 研究领域：PARP inhibition in cancer therapy、DNA Repair Mechanisms、RNA Research and Splicing

Poly(ADP-ribose) polymerase 1 (PARP1) detects DNA strand breaks that occur in duplex DNA and chromatin. Here, correlative optical tweezers and fluorescence microscopy reveal how single molecules of PARP1 identify single-strand breaks (i.e., nicks), undamaged nucleosome core particles (NCP) and NCPs containing DNA nicks. Fluorescently-tagged PARP1 or PARP2 from nuclear extracts binds nicks with nanomolar affinity but does not engage undamaged dsDNA regions. In contrast, PARP1 avidly binds undamaged NCPs, and partial NCP unwrapping induced by DNA tension significantly increases PARP1 on rate and affinity. Catalytically dead PARP1 or EB-47 inhibition greatly increases PARP1 affinity to DNA nicks and undamaged NCP, implicating a mechanism where PARP1 reverse allostery regulates PARP1 retention to undamaged chromatin. We also monitor ADP-ribosylation in real time upon PARP1 binding undamaged or nicked NCPs. These results provide key mechanistic insights into domain allostery and how pharmacological intervention alters PARP1 binding dynamics for therapeutic impacts. Poly-ADP-ribose polymerase 1 (PARP1) plays key roles in DNA repair, transcription, and replication. Here, the authors used a single-molecule approach to reveal how PARP1 identifies DNA single-strand breaks in nucleosomes and how PARP1 activity regulates its DNA and chromatin binding kinetics.