Nanoparticle retention enables non-invasive detection of metastases by magnetic particle imaging in murine breast cancer models

作者：Preethi Korangath, Hayden Carlton, Toby Sanders, Olivia C. Sehl, Suqi Ke, Abdul Rahman Mohtasebzadeh, Lyndsey Werhane, Cordula Grüttner, Chen Hu, Kathleen Gabrielson, Patrick Goodwill, Robert Ivkov · 发表于：Theranostics · 年份：2025 · DOI：10.7150/thno.122259 · 被引用次数：3 · 研究领域：Characterization and Applications of Magnetic Nanoparticles、Geomagnetism and Paleomagnetism Studies、Nanoparticle-Based Drug Delivery

Early detection of metastatic disease improves cancer survival, yet existing modalities are limited in their detection capabilities.We propose that magnetic particle imaging (MPI), an emerging technology, can be used for early detection of primary tumors and metastases.MPI detects minute quantities of magnetic particles that act as "cold tracers" which accumulate in areas of high immune activity.Methods: Pegylated Synomag® nanoparticles were intravenously injected into mouse models of breast cancer bearing primary tumors and spontaneously developed lung metastases.After 72 h, mice were subjected to three-dimensional MPI followed by structural imaging for co-registration.Non-tumor bearing mice served as controls for background signal correction and toxicity analysis.Animals were then sacrificed to collect tumors and organs of interest for two-dimensional MPI scans before fixing them for histopathological evaluation by hematoxylin and Eosin (H&E), Prussian blue, and immunohistochemistry staining.To further substantiate our findings towards clinical translation, tumor phantoms with nanoparticles were evaluated in a newly-built human scale MPI.Results: Pegylated Synomag® nanoparticles showed a strong signal in both in vitro and in vivo models.Multiple macro and micro metastatic sites were identified by MPI and later confirmed by histology.Ex vivo quantitative analysis showed MPI can detect metastasis with high specificity and sensitivity, with positive correlations between tumor ...