Molecular imaging of fibroblast activation in systemic sclerosis using [18F]AlF-NOTA-FAPI-04 PET/CT: a prospective cohort study

作者：Meixin Zhao, Hui Wei, Meng Wang, Zeyang Wang, Xinwei Li, Hui Li, Annan Zhang, Song Xue, Marcus Hacker, Weifang Zhang, Rong Mu, Xiangyang Li · 发表于：European Journal of Nuclear Medicine and Molecular Imaging · 年份：2025 · DOI：10.1007/s00259-025-07586-4 · 被引用次数：5 · 研究领域：Peptidase Inhibition and Analysis、Bone and Dental Protein Studies、Protein Tyrosine Phosphatases

This prospective cohort study evaluated the utility of [¹⁸F]AlF-NOTA-FAPI-04 PET/CT for whole-body assessment of fibroblast activation in systemic sclerosis (SSc), correlating organ-specific uptake with clinical indicators of severity and multi-organ dysfunction. 31 patients with SSc and 21 patients from a non-SSc cohort underwent total-body [¹⁸F]AlF-NOTA-FAPI-04 PET/CT imaging. Quantitative uptake parameters were measured in lungs, heart, kidneys, skeletal muscles, esophagus, and skin. Clinical assessments were completed within ± 14 days. Inter-organ metabolic correlations were analyzed. Abnormally elevated FAPI uptake was prevalent in the lungs (96.8%), heart (35.5%), kidneys, and skeletal muscles (12.9%). Compared with the non-SSc cohort, patients with SSc exhibited significantly elevated whole lung SUVmean(wlSUVmean:overall adjusted P-value(padj) < 0.001) and cardiac SUVmax (cSUVmax: median1.37, IQR 1.17–1.87 vs.1.01 ± 0.15, padj <0.001). Reduced pulmonary function showed significant negative correlations with wlSUVmean (padj <0.01). SSc patients with renal impairment demonstrated markedly higher renal SUVmax(rSUVmax)and renal SUVmean (rSUVmean) compared to both SSc patients with preserved renal function and the non-SSc cohort(both overall padj < 0.001). Muscular uptake aligned with elevated creatine kinase and biopsy-proven myopathy. No significant tracer accumulation was observed in the esophagus or skin. Inter-organ correlation analysis revealed metabolic coupling acro...