Highly Efficient Multiplex Precise Genome Editing in Porcine Cells Using epiCBE4max System

作者：Xianghua Xu, Bo Li, Jing Lü, Xuewen Xu, Shengsong Xie, Changchun Li, Xinyun Li, Xiaosong Han, Jinxue Ruan · 发表于：Journal of Integrative Agriculture · 年份：2025 · DOI：10.1016/j.jia.2025.12.011 · 被引用次数：1 · 研究领域：CRISPR and Genetic Engineering、RNA regulation and disease、Virus-based gene therapy research

1. EpiCBE4max, a high-efficiency, one-step multiplex base editing system, was developed by integrating episomal elements with the CBE4max platform. 2. The epiCBE4max system achieved a simultaneous homozygous editing efficiency of 44.44% across five target genes in porcine fetal fibroblasts with undetectable off-target effects.