Assembly and analysis of the complete mitochondrial genome of endangered plant Tilia amurensis Rupr

作者：Xiao Liu, Furong Lin, Shasha Zhai, Zhicheng Wu, Lei Gao, Nana Sun, Wenchao Yan, Jitong Chen, Dan Liu · 发表于：Frontiers in Plant Science · 年份：2025 · DOI：10.3389/fpls.2025.1686403 · 被引用次数：1 · 研究领域：Photosynthetic Processes and Mechanisms、Mitochondrial Function and Pathology、Genomics and Phylogenetic Studies

Introduction Tilia amurensis Rupr., a deciduous tree of the genus Tilia in the Malvaceae family, is classified as a second-class key protected wild plant in China. This study aimed to sequence and analyze the complete mitochondrial genome of T. amurensis to (i) aid the understanding of phylogenetic relationships within the Malvaceae family, (ii) clarify the taxonomic status of genus Tilia , and (iii) provide a molecular basis for the conservation and genetic breeding of this endangered species. Methods T. amurensis mitochondrial genome was sequenced and assembled using the Illumina and Oxford Nanopore Technologies platforms. Additionally, bioinformatics analyses were conducted, along with gene annotation and functional analysis, to identify repeat sequences and chloroplast-derived fragments. Results The assembled mitochondrial genome of T. amurensis was found to be a single circular molecule with a total length of 830,088 bp and a GC content of 44.97%. A total of 61 genes were annotated, including 20 tRNA, 3 rRNA, and 38 protein-coding genes. Moreover, 472 RNA editing sites were identified among the protein-coding genes. We also detected a high abundance of repetitive sequences and chloroplast-derived fragments. Specifically, the genome included 264 simple sequence repeats, 19 tandem repeats, and 855 pairs of scattered repeats, predominantly comprising short fragment repetitions. Furthermore, 22 chloroplast homologous fragments (with a cumulative length of 8,138 bp) were iden...