Jiawei Buyang Huanwu Decoction modulates gut microbiota and metabolic profiles in a rat model of idiopathic pulmonary fibrosis

作者：Dandan Sun, Xinxin Cao, Jing Sun, Huimin Zhang, Yujun Liu · 发表于：Frontiers in Microbiology · 年份：2025 · DOI：10.3389/fmicb.2025.1704103 · 被引用次数：1 · 研究领域：Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis、Rheumatoid Arthritis Research and Therapies、Chronic Obstructive Pulmonary Disease (COPD) Research

Introduction: Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease with irreversible fibrosis and poor prognosis. Jiawei Buyang Huanwu Decoction (JBHD) has demonstrated therapeutic effects, but the exact mechanisms, particularly those mediated by the gut microbiota, remain largely unexplored. This study aimed to explore how JBHD modulates gut microbiota, and how these changes may influence host metabolic regulation in the context of IPF. Methods: The IPF model was established via intratracheal bleomycin injection. After 28 days of treatment, feces samples were obtained for 16S rRNA gene sequencing, whereas serum and urine samples were collected for metabolomic analyses. Results: , and Christensenellaceae R-7 group at the genus level. The microbial functions reversed by JBHD in both KEGG Level 3 and COG analyses were related to amino acid metabolism, including Biosynthesis of amino acids (KEGG) and Amino acid transport and metabolism (COG). Serum and urine metabolomics showed that JBHD modified the metabolic profile of IPF rats. Among the differentially expressed metabolites (DEMs) altered by IPF, JBHD reversed 11 in serum and 13 in urine. Pathway analysis indicated that these DEMs were mainly associated with amino acid and lipid metabolism. The consistency between microbial functional predictions and host metabolomic findings in amino acid metabolism suggests that JBHD may influence host metabolic pathways through gut microbiota modulation. Functional prediction ...