Development of a light-induced gas phase nitric oxide generator and its use in killing biofilm bacteria in vitro and ex vivo

作者：Gergely Lautner, Partha Sarathi Sheet, Mohsen Tabasi, Haleh Ganijan, Hannah J. Naldrett, Blake Stringer, Jeremy Schwartz, Joshua C. Doverspike, Denis Hadjiliadis, Jiaqi Wu, Chuanwu Xi, Mark E. Meyerhoff, Umadevi Sajjan, Steven P. Schwendeman · 发表于：Journal of Controlled Release · 年份：2025 · DOI：10.1016/j.jconrel.2025.114483 · 被引用次数：3 · 研究领域：Bacterial biofilms and quorum sensing、Cystic Fibrosis Research Advances、Inhalation and Respiratory Drug Delivery

In order to overcome the high costs and logistical issues associated with nitric oxide (NO) storage tanks, a portable tankless prototype device has been developed for precise controlled release of inhaled nitric oxide (iNO) gas from a solid NO donor, S -nitroso- N -acetyl penicillamine (SNAP) stored within “coffee pod” style replaceable cartridges. The device utilizes LED light to trigger the release of NO from SNAP, and the concentration of the delivered NO gas is continuously monitored with amperometric sensors and regulated by a feedback control system via modulating the light intensity. The system enables facile adjustment of NO concentrations, allowing for the reliable delivery of low-dose NO (up to 10 ppm, as demonstrated here) across clinically relevant air flow rates, with the prototype specifically designed for air flow up to 4 L/min. Importantly, NO generation is achieved without the need for nitrogen (N₂) as a carrier gas, enabling safe and simple operation using ambient air even in home settings. To ensure the safety of this device, the key factors affecting the formation of the major undesired impurity, nitrogen dioxide (NO 2 ), during NO generation were carefully considered and minimized to levels suitable for NO inhalation therapy (<1 ppm). The gas phase NO generated by this device significantly reduced the biofilm density as measured by crystal violet staining and the viable bacterial counts in the mucoid P. aeruginosa biofilm established on a plastic surface ...