Mechanism Of Action Of Linezolid-Mediated Erk/Mapk Pathway In Bone Repair Of Rats With Chronic Osteomyelitis Caused By Staphylococcus Aureus Infection

作者：Jinlong Zheng, A. Y. Chen, Jing Li · 发表于：Acta Poloniae Pharmaceutica - Drug Research · 年份：2025 · DOI：10.32383/appdr/208852 · 研究领域：Antimicrobial Resistance in Staphylococcus、Bone Metabolism and Diseases、Osteomyelitis and Bone Disorders Research

Background: chronic osteomyelitis has become a clinical challenge due to bacterial resistance and uncontrolled inflammation. Staphylococcus aureus is the most common pathogen, but the changes in the extracellular regulated kinase/microtubule-associated protein kinase (ERK/MAPK) signaling pathway and the intervention mechanism of linezolid after infection remain unclear. Objective: it was to investigate the therapeutic effect of linezolid on Staphylococcus aureus-induced chronic osteomyelitis and its regulatory role in the ERK/MAPK pathway. Methods: forty rats were randomly divided into control group (CG), sham group (SG), model group (MG), and drug group (DG). A tibial Staphylococcus aureus infection model was established, and the DG received linezolid treatment for 28 days post-surgery. Infection severity was assessed using Rissing and Norden scores; serum levels of interleukin (IL)-1, IL-1β, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, IL-10, and C-reactive protein (CRP) were measured; bacterial load in bone tissue was quantified. Results: In the MG, Rissing and Norden scores increased over time but significantly decreased in the DG. The MG showed elevated IL-1, IL-1β, TNF-α, IFN-γ, and CRP, with reduced IL-10, while the DG exhibited the opposite trend. Bacterial load in bone tissue reached 8.92–10.33×104 CFU/g in the MG but decreased to 1.42–5.08×104 CFU/g in the DG. The MG had reduced p-ERK1/2 and p-MEK1/2 expression, which was significantly restored in the DG (all ...