Nanopeptides-Loaded Electro-Assembled Oriented Collagen Matrix for the Synergistically Promotes Early Angiogenesis in Tendon Healing

作者：Han Gao, Chengxuan Yu, Xiner Du, Zixuan Zhang, Lei Miao, Hao Luo, Huaixuan Sheng, Jian Zhang, Yu Dong, Yunxia Li, Sijia Feng, Shiyi Chen, Dandan Sheng, Xue Qu, Jun Chen · 发表于：ACS Nano · 年份：2025 · DOI：10.1021/acsnano.5c09232 · 被引用次数：3 · 研究领域：Tendon Structure and Treatment、Periodontal Regeneration and Treatments、Angiogenesis and VEGF in Cancer

Angiogenesis is a critical process in the early stages of tendon healing but is always limited by its avascular structure, thus impairing effective healing. Nanopeptides targeting endogenous vascular endothelial growth factor (VEGF) presented a promising strategy for promoting early angiogenesis in tendon healing. In this study, we introduced a nanoscale electro-assembly technique to kinetically control the collagen assembling to form VEGF-binding protein (VEGF-BP) and PR1P-loaded oriented collagen matrix (BP/P Col) for tendon healing. The specific binding of VEGF-BP and PR1P to endogenous VEGF mediated the proangiogenic effects during the early stages of repair. In vitro cell experiments showed that BP/P Col could promote human umbilical vein endothelial cell migration and tube formation as well as new vessel formation in chick chorioallantois. In a rabbit model of tendon defect, it increased vascular area and new vessel formation within the first 2 weeks. Additionally, multiomics analysis further revealed the potential mechanism of BP/P Col in promoting early angiogenesis was to modulate the macrophage-regulated VEGF expression, providing positive feedback for early angiogenesis. This process induced the organized collagen structure along the oriented collagen matrix, which ultimately facilitated rapid tendon healing during the early stage. Overall, this study demonstrated that BP/P Col, through the efficient loading of VEGF-BP and PR1P, exerted a synergistic enhancement ef...