Synergistic Catalysis of Gold–Platinum Alloy Nanozymes: A Novel Colorimetric Sensor for ALP Detection in Complex Biological Matrices

作者：Baojie Du, Bingqing Zhang, Xiaofeng Ren, Jie Yang, Fan Yang, Chunyu Yan, Liping Li, Ruiping Zhang · 发表于：Pharmaceuticals · 年份：2025 · DOI：10.3390/ph18121795 · 被引用次数：2 · 研究领域：Advanced Nanomaterials in Catalysis、Carbon and Quantum Dots Applications、Nanocluster Synthesis and Applications

Background/Objectives: Alkaline phosphatase (ALP) is a crucial enzyme in numerous pathological processes and a significant biomarker in clinical diagnostics. Conventional ALP detection methods are hampered by reliance on complex sample pretreatment, sophisticated instrumentation, time-consuming procedures, and high costs. This study aimed to develop a simple, rapid, and cost-effective colorimetric sensing method for ALP detection with enhanced resistance to matrix interference in biological samples. Methods: We designed a colorimetric assay based on bimetallic gold–platinum nanocatalysts (AuPt NPs) exhibiting peroxidase-like (POD-like) activity. The detection principle involves a dual-reaction cascade: (1) Alkaline phosphatase (ALP) catalyzes the conversion of trisodium L-ascorbic acid-2-phosphate (AA2P) into ascorbic acid (AA), and (2) the generated AA reduces oxidized 3,3′,5,5′-tetramethylbenzidine (oxTMB) produced by the catalytic activity of AuPt NPs. This method was evaluated for its detection performance in diluted human serum without complex sample pretreatment. Results: AuPt NPs exhibited resistance to biological matrix interference, enabling sensitive detection of ALP. The assay showed a linear ALP detection range of 0–90 mU·mL−1 (R2 = 0.994) and a limit of detection of 3.91 mU·mL−1. In spiked human serum, recoveries were 95.45–111.97%, with negligible interference from ions and biomolecules. Conclusions: We developed a simple, rapid, and reliable colorimetric sensor...