UGGT1 mediates N-glycosylation of LGALS3BP to induce angiogenesis in liver cancer via the NOTCH signaling pathway

作者：Bixiu He, Liang Wang, Jiadong Xia, X. Xu, Jianguo Xu, Geqiong Xiao, Jiamin Sheng · 发表于：Clinical Proteomics · 年份：2026 · DOI：10.1186/s12014-026-09624-1 · 研究领域：Glycosylation and Glycoproteins Research、Galectins and Cancer Biology、Microbial metabolism and enzyme function

Abstract Background Liver cancer (LC) is one of the leading causes of cancer-related deaths worldwide. Its complex vascular regulatory network and drug resistance severely limit clinical efficacy. Although LGALS3BP is highly expressed in various malignant tumors, its biological function and molecular regulatory mechanisms in LC angiogenesis remain to be elucidated. Methods Bioinformatics analysis was used to examine the expression of LGALS3BP in hepatocellular carcinoma (HCC) and its relationship with angiogenesis and the NOTCH signaling pathway. qRT-PCR, Western blot (WB), and ELISA were performed to validate the mRNA, protein expression, and secretion levels of LGALS3BP. The effects of LGALS3BP on HCC cell viability, proliferation, and invasion were assessed using CCK-8, colony formation, and Transwell assays. A co-culture system of HCC cells and human umbilical vein endothelial cells was established to evaluate pro-angiogenic effects. qRT-PCR and WB were used to detect mRNA and protein expression of the NOTCH pathway. Bioinformatics analysis combined with Co-IP and IF validated the interaction between UGGT1 and LGALS3BP. N-glycosylation sites of LGALS3BP were analyzed, and the role of UGGT1 in LGALS3BP N-glycosylation was investigated through protein stability assays, WB, and ELISA. The impact of UGGT1-mediated LGALS3BP N-glycosylation on the NOTCH pathway and HCC angiogenesis was explored via qRT-PCR, WB, functional cell assays, and co-culture experiments. Finally, in viv...