Deep Learning-Based Prediction of Enzyme Optimal pH and Design of Point Mutations to Improve Acid Resistance

作者：Sizhe Qiu, Nan‐Kai Wang, Yishun Lu, Jin-Song Gong, Jin‐Song Shi, Aidong Yang · 发表于：ACS Synthetic Biology · 年份：2025 · DOI：10.1021/acssynbio.5c00679 · 被引用次数：7 · 研究领域：Enzyme Catalysis and Immobilization、Microbial Metabolic Engineering and Bioproduction、Enzyme Structure and Function

High Resolution Image Download MS PowerPoint Slide An accurate deep learning predictor of enzyme optimal pH is essential to quantitatively describe how pH influences the enzyme catalytic activity. CatOpt, developed in this study, outperformed existing predictors of enzyme optimal pH (RMSE = 0.833 and R 2 = 0.479), and could provide good interpretability with informative residue attention weights. The classification of acidophilic and alkaliphilic enzymes and prediction of enzyme optimal pH shifts caused by point mutations showcased the capability of CatOpt as an effective computational tool for identifying enzyme pH preferences. Furthermore, a single point mutation designed with the guidance of CatOpt successfully enhanced the activity of Pyrococcus horikoshii diacetylchitobiose deacetylase at low pH (pH = 4.5/5.5) by approximately 7%, suggesting that CatOpt is a promising in silico enzyme design tool for pH-dependent enzyme activities.