Comprehensive Assessment of Silver Bioaccumulation and DNA Damage Effects in Coturnix coturnix japonica via Blood, Feather, and Egg Using Two Different Sources

作者：Hanan Al‐Khalaifah, Nudrat Fatima, Shabana Naz, Babar Maqbool, Rifat Ullah Khan, Ankqash Ayyub, Muhammad Usama, Swaira Ashfaq, Hifza Shehzadi, Sania Satti, Alaeldein M. Abudabos, Ibrahim A. Alhidary · 发表于：Animals · 年份：2025 · DOI：10.3390/ani15233370 · 被引用次数：5 · 研究领域：Nanoparticles: synthesis and applications、Heavy Metal Exposure and Toxicity、Mercury impact and mitigation studies

This study examined the effects of Ag-NPs and AgNO3 on silver accumulation in the blood, feathers, eggshells, and egg contents of Japanese quails, as well as their potential to cause DNA damage. A total of 480 (fourteen-day-old) quails were divided into five groups of 96 birds each, arranged into six replicates of sixteen birds with a sex ratio of four males to twelve females. Birds were housed in cages measuring 120 × 60 × 50 cm, and the trial lasted for 65 days. At the end of the trial, six birds per replicate (36 birds per group) were randomly selected and slaughtered for sample collection. Blood was collected via wing vein puncture, feathers were plucked from the breast region, and eggs were collected daily for analysis of eggshell and internal contents. The first group served as a control and was fed a basal diet, while the second and third groups received Ag-NPs at doses of 10 mg/kg and 20 mg/kg, respectively. The fourth and fifth groups were given AgNO3 at the same concentrations. The results showed that the highest silver accumulation occurred in all tissues in quails fed the higher dose of Ag-NPs. The greatest accumulation was observed in the eggshells, likely due to their porous structure, which facilitates metal deposition. Both Ag-NPs (20 mg/kg) and AgNO3 (10 and 20 mg/kg) induced DNA damage, although the damage was more severe in the groups exposed to Ag-NO3. A positive correlation was observed between treatment groups and comet assay parameters, indicating incre...