Investigation of Antimicrobial Potential of Medicinal Plants Against Pseudomonas aeruginosa

作者：Nosheen Bibi, Shazia Perveen, Sumaira Kanwal, Fariha Latif, Rehmana Rashid, Sara Janiad, Iram Qadeer, Fatima Naseem, Abdullah R. Alanzi, Rashed N. Herqash, Imran Haider, Mehraj Abbasov, Sadaf Kayani, Mohd Asif Shah · 发表于：Food Science & Nutrition · 年份：2025 · DOI：10.1002/fsn3.70999 · 被引用次数：2 · 研究领域：Essential Oils and Antimicrobial Activity、Silymarin and Mushroom Poisoning、Phytochemistry Medicinal Plant Applications

ABSTRACT Pseudomonas aeruginosa , a gram‐negative bacterium present in water and soil, causes significant illnesses and chronic conditions that degrade drinking water quality. This study aimed to evaluate the antibacterial and antioxidant activities of plant extracts ( Convolvulus arvensis , Chenopodium murale , Avena fatua , Cirsium arvense , and Hordeum vulgare ) against P. aeruginosa . FTIR spectroscopy was used to investigate in the plant extracts, and the phytochemical compounds found in the plant extracts were analyzed using GC–MS analysis. This study evaluated the antioxidant activity of plant extracts using DPPH scavenging assays and found that ascorbic acid significantly improved the DPPH scavenging activity of C. arvensis extract compared to that of other plants. Saliva samples were collected from the patients to differentiate P. aeruginosa from other oral cavity microorganisms. Gram staining and catalase testing were performed to identify bacterial strains. Pseudomonas aeruginosa produces a positive catalase test and appears as a pink or red rod after Gram staining. This study revealed that five plant extracts have antibacterial activity against P. aeruginosa. Cirsium arvensis showed a higher zone of inhibition (72 mm). The MIC was the lowest concentration that inhibited the visible growth of a bacterium, whereas the MBC was the lowest concentration that killed 99.9% of the bacteria. A. fatua and C. murale were effective at very low concentrations, inhibiting bacte...