A transcriptomic atlas of astrocyte heterogeneity across space and time in mouse and marmoset

作者：Margaret E. Schroeder, Dana McCormack, Lukas R. Metzner, Jinyoung Kang, Kenneth K. W. Li, Eunah Yu, Lisa Melamed, Kirsten Levandowski, Heather Zaniewski, Qiangge Zhang, Edward S. Boyden, Fenna M. Krienen, Guoping Feng · 发表于：Neuron · 年份：2025 · DOI：10.1016/j.neuron.2025.09.011 · 被引用次数：23 · 研究领域：Neurogenesis and neuroplasticity mechanisms、Neuroinflammation and Neurodegeneration Mechanisms、Neuroscience and Neuropharmacology Research

How astrocyte regionalization unfolds over development is not fully understood. We used single-nucleus RNA sequencing to characterize the molecular diversity of brain cells across six developmental stages and four brain regions in the mouse and marmoset brain. Our analysis revealed striking regional heterogeneity among astrocytes, particularly between telencephalic and diencephalic regions in both species. Most of the region patterning was private to astrocytes and not shared with neurons or other glial types. Though astrocytes were already regionally patterned in late embryonic stages, this region-specific astrocyte gene expression signature changed significantly over postnatal development, and its composition suggests that regional astrocytes further specialize postnatally to support their local neuronal circuits. Across mouse and marmoset, we found hundreds of species-differentially expressed genes and divergence in the expression of astrocytic region- and age-differentially expressed genes. Finally, we used expansion microscopy to show that astrocyte morphology is also regionally specialized.