Screening of several evaluation indicators for alkali resistance in fish

作者：Xuefei Zhao, Jing Huang, Yuting Zou, Kexin Zhang, Zhipeng Sun, Limin Zhang, Yanchun Xu, Yu‐Mei Chang · 发表于：Fish & Shellfish Immunology · 年份：2025 · DOI：10.1016/j.fsi.2025.111010 · 被引用次数：1 · 研究领域：Physiological and biochemical adaptations、Aquaculture Nutrition and Growth、Aquaculture disease management and microbiota

The vast expanse of saline-alkali waters worldwide poses significant challenges to fish survival. To identify key evaluation indicators for fish alkali tolerance and guide the selection of new alkali-tolerant fish varieties, this study selected the alkali forms of Crucian carp and Amur ide from Dali Lake (50mM alkalinity) as research subjects. These alkali and freshwater populations underwent 7-day alkalinity stress treatments (10, 30, 50 mM), with systematic monitoring of hematological and serum biochemical indicators. Results showed that as alkalinity increased, the fish initiated corresponding immune responses, with Crucian carp showing a significantly stronger response than Amur ide. Furthermore, the freshwater forms of both fish species were more significantly affected by the external environment compared to their alkali forms, as evidenced by the significant increases in white blood cell count and neutrophil count in the high-alkalinity groups to varying degrees. Meanwhile, erythrocyte parameters rose significantly under high alkalinity, with Crucian carp exhibiting a more pronounced increase, indicating that fish respond to harsh environments by enhancing their oxygen transport efficiency. Additionally, changes in the serum concentrations of Na + and Cl - ions reflected differences in the osmoregulatory capacity of the fish, with both alkali forms fish species demonstrating stronger overall ion regulatory abilities. In high-alkaline environments, fish also exhibited im...