Light-induced extracellular vesicle and particle adsorption

作者：Colin L. Hisey, Xilal Y. Rima, Jacob Doon‐Ralls, Chiranth K. Nagaraj, Sophia Mayone, Kim Truc Nguyen, Sydney Wiggins, Kalpana Deepa Priya Dorayappan, Xin Huang, Mangesh Dattu Hade, Karuppaiyah Selvendiran, James N. Higginbotham, Oleg S. Tutanov, Jeffrey L. Franklin, Robert J. Coffey, David A. Wood, Chunyu Hu, Divya S. Patel, Setty M. Magaña, Andre Francis Palmer, Derek J. Hansford, Eduardo Reátegui · 发表于：Nature Methods · 年份：2025 · DOI：10.1038/s41592-025-02914-w · 被引用次数：5 · 研究领域：Extracellular vesicles in disease、Nanoplatforms for cancer theranostics、Neutrophil, Myeloperoxidase and Oxidative Mechanisms

The role of extracellular vesicles (EVs) and particles (EPs/EVPs) in human health and disease has garnered considerable attention over the past two decades. However, while several types of EVPs are known to interact dynamically with the extracellular matrix and there is great potential value in producing high-fidelity EVP micropatterns, there are currently no label-free, scalable and tunable platform technologies with this capability. We introduce light-induced extracellular vesicle and particle adsorption (LEVA) as a powerful solution to study surface-bound EVPs. The versatility of LEVA is demonstrated using GFP-EV standards, EVs from conventional and bioreactor cultures, DiFi exomeres and Escherichia coli EVs, with the resulting patterns used for single-EV fluorescence imaging, cell migration on migrasome-mimetic trails and bacterial EV-mediated neutrophil swarming. LEVA will rapidly advance our understanding of extracellular matrix protein- and surface-bound EVPs and should encourage researchers from many disciplines to create new biomimetic, immunoengineering and other assays.