Chemical constituents and biological activities of the endophytic fungus Hypoxylon sp. from Cupressus funebris Endl

作者：Yun-He Tuo, Zhong‐Duo Yang, Bing Sun, Cheng‐Yuan Xu, Jianguo Chen, Tiankun Zhao, Ai-Hong Zhao, Sahyun Kim · 发表于：Natural Product Research · 年份：2025 · DOI：10.1080/14786419.2025.2588801 · 研究领域：Microbial Natural Products and Biosynthesis、Synthetic Organic Chemistry Methods、Fungal Biology and Applications

Four compounds, Hypoxylonines A-D (1–4), were isolated from the ethyl acetate extract of the endophytic fungus Hypoxylon sp. derived from Cupressus funebris Endl., among them, Hypoxylonine A (1) is a previously unreported compound, although compounds 2–4, known from synthetic studies, are reported here for the first time as natural products. These compounds are structurally linked by a ketone carbonyl group to a toluene group and a cyclopentane unit. The structures of compounds 1–4 were elucidated by IR, HRESIMS, and one-dimensional and two-dimensional nuclear magnetic resonance (NMR) spectra. The NMR data of the new natural products (compounds 2–4) being reported for the first time. These compounds were evaluated for their inhibitory activities against phosphodiesterase 1 (PDE1) and Fusarium graminearum Schwabe. The results showed that compound 3 had strong inhibitory activity against PDE1 at a concentration of 100.0 μg/mL, with an inhibition rate of 78.70 ± 1.07% and an IC50 value of 40.78 ± 0.12 μg/mL.