Stromal vascular fraction self-assembles vascularized osteogenic organoids with immunomodulatory functions

作者：Jiazhou Wu, Ying He, Tao Qian, Zexian Liu, Yanbin Wu, Yazhou Li, Hongyu Jiang, Jianting Ye, Jia Lv, Biao Ma, En Luo, Jialiang You, Dingkai Wang, Yun Bai, Junming Zhang, Liang Zuo, Jiang Peng · 发表于：Bioactive Materials · 年份：2025 · DOI：10.1016/j.bioactmat.2025.10.030 · 被引用次数：4 · 研究领域：Mesenchymal stem cell research、3D Printing in Biomedical Research、Immune cells in cancer

Bone tissue engineering has enormous potential for treating complex orthopedic conditions such as Large Bone Defects (LBDs), delayed union or non-union fractures, and Osteonecrosis (ON). On the other hand, the adipose tissue-derived Stromal Vascular Fraction (SVF) comprises a heterogeneous population of cells, including Adipose-Derived Mesenchymal Stem Cells (ADSCs), Endothelial Progenitor Cells (EPCs), pericytes, and Hematopoietic Stem Cells (HSCs). Owing to its ease of acquisition, abundance, and the intrinsic involvement of its cellular components in bone repair, the SVF is presently considered a viable source for constructing osteogenic organoids. Herein, we leveraged the self-assembly capacity of SVF cells to construct spatially complex organoids with osteogenic, angiogenic, and immunomodulatory properties that mimic native tissues. Compared to spheroids from ADSCs alone, SVF cells exhibited natural tissue-like spatial organization, as well as superior osteogenic, angiogenic, and immunomodulatory potential, after two weeks of 3D spheroid culture. Furthermore, SVF-derived organoids showed distinct spatial architectures and functional profiles under both the growth and osteogenic induction conditions. Proteomic analysis and cytokine array profiling further revealed that SVF organoids were functionally enriched in the cytokine signaling and inflammatory/immune regulation pathways, with greater Extracellular Matrix (ECM) production observed under growth conditions compared t...