Akkermansia muciniphila and its culture supernatant ameliorate colitis in interleukin-10 knockout mice via gut barrier and immune modulation

作者：Mingshan Jiang, Yongbin Jia, Chunxiang Ma, Zhen Zeng, Y P. Li, Lili Li, Hao Lin, Jiangmei Pang, Jiaxin Li, Jingjing Chen, Xi Li, Kexin Chen, Hong Zhang, Hong Zhang, Yu Gan, Zou Xiang, Linlin Chen, Hu Zhang, Hu Zhang · 发表于：Frontiers in Immunology · 年份：2025 · DOI：10.3389/fimmu.2025.1693007 · 被引用次数：10 · 研究领域：Gut microbiota and health、Inflammatory Bowel Disease、Probiotics and Fermented Foods

Background Akkermansia muciniphila ( A. muciniphila ) is a mucin-degrading commensal bacterium with established roles in maintaining intestinal homeostasis and modulating host immune responses. However, its therapeutic potential and mechanisms in chronic spontaneous colitis remain incompletely defined. Interleukin-10 knockout (IL-10 − / − ) mice are a well-established model of spontaneous colitis due to impaired anti-inflammatory cytokine signaling and disrupted immune regulation. In this study, we aimed to determine whether A. muciniphila and its culture-derived supernatant could ameliorate intestinal inflammation in IL-10 − / − mice. Methods We administered A. muciniphila or its culture supernatant ( A. muciniphila supernatant [A.m-SN]) to IL-10 − / − mice and monitored disease progression over 30 days. Clinical assessments included body weight, disease activity index (DAI), colon length, histopathology, and endoscopy. Tight junction proteins (zonula occludens-1 [ZO-1] and Occludin) and colonic messenger ribonucleic acid levels of pro- and anti-inflammatory cytokines were evaluated. Gut microbiota composition was assessed using 16S ribosomal deoxyribonucleic acid sequencing, and untargeted metabolomic profiling was performed on the culture medium to identify potential anti-inflammatory components. Results Mice treated with A. muciniphila or A.m-SN showed significantly reduced colitis severity, including reduced body weight loss, lower DAI, preserved colon length, and improv...