Distinct transcriptomic and epigenomic responses of mature oligodendrocytes during disease progression in a mouse model of multiple sclerosis

作者：Chao Zheng, Bastien Hervé, Mandy Meijer, Leslie A. Kirby, André Ortlieb Guerreiro‐Cacais, Petra Kukanja, Mukund Kabbe, Tony Jimenez-Beristain, Tomas Olsson, Eneritz Agirre, Gonçalo Castelo‐Branco · 发表于：Nature Neuroscience · 年份：2025 · DOI：10.1038/s41593-025-02100-3 · 被引用次数：11 · 研究领域：Neurogenesis and neuroplasticity mechanisms、Multiple Sclerosis Research Studies、Neuroinflammation and Neurodegeneration Mechanisms

Multiple sclerosis (MS) is a chronic autoimmune disease that targets mature oligodendrocytes (MOLs) and their myelin. MOLs are heterogeneous and can transition to immune-like states in MS. However, the dynamics of this process remain unclear. Here, we used single-cell multiome assay for transposase-accessible chromatin and RNA sequencing targeting oligodendroglia (OLG) from the experimental autoimmune encephalomyelitis (EAE) MS mouse model at multiple disease stages. We found that immune OLG states appear at early disease stages and persist to late stages, which can be consistent with epigenetic memory of previous neuroinflammation. Transcription factor activity suggested immunosuppression in OLG at early disease stages. Different MOLs exhibit differential responsiveness to EAE, with MOL2 exhibiting a stronger transcriptional immune response than MOL5/MOL6, and showed divergent responses at the epigenetic level during disease evolution. Our single-cell multiomic resource highlights dynamic and subtype-specific responses of OLG to EAE, which might be amenable to modulation in MS.