Approaching single-molecule assembly-free readout from medium-length encoded DNA

作者：Weigang Chen, Rui Qin, Quan Guo, Jian Guo, Qi Ge, Ying‐Jin Yuan · 发表于：Nature Communications · 年份：2025 · DOI：10.1038/s41467-025-65004-7 · 被引用次数：3 · 研究领域：DNA and Biological Computing、Nanopore and Nanochannel Transport Studies、Advanced biosensing and bioanalysis techniques

For DNA data storage, nanopore sequencing can facilitate rapid readout but suffers from severe insertion/deletion errors, which are quite computationally expensive to correct. Here, we propose a nearly single-molecule and assembly-free readout scheme for medium-length pseudo-noise piloting DNA fragments. Specifically, we devise medium-length DNA fragments using low-density parity-check codes companioned by pseudo-noise sequence (PNC-LDPC). A single cleavage on this encoded DNA by transposase generates DNA fragments of approximately full length. Using the readout-aware pseudo-noise sequences, noisy nanopore reads with arbitrary start points are directly located, and base insertions/deletions are corrected, enabling fast and reliable recovery even at very low coverages. Experimental results indicate that the data can be reliably recovered at a coverage of 1.24–3.15× with a typical nanopore sequencing error rate of 1.83%. This method enables error-free recovery in near single-molecule scenarios, highlighting the potential of PNC-LDPC encoded medium-length DNA for data storage applications. Nanopore sequencing offers rapid DNA readout but suffers from severe insertion/deletion errors. Here, authors devise medium-length DNA fragments using a PNC-LDPC coding scheme, with an efficient cleavage library preparation to quickly recover original data at very low coverages without assembly.