Jinbei Decoction Attenuates LPS‐Induced Acute Lung Injury via Suppression of TRAF6‐Dependent Inflammatory Response in Macrophage

作者：Wei Li, Aijun Zhang, Yongqing Cai, Haoyu Sun, Yao Teng, Zhaoqing Meng, Weiwei Zhou, Ruixin Liu, Zhen Zhang, Jingzhen Tian, Xia Li · 发表于：Journal of Cellular and Molecular Medicine · 年份：2025 · DOI：10.1111/jcmm.70944 · 被引用次数：4 · 研究领域：Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis、NF-κB Signaling Pathways、Andrographolide Research and Applications

Acute lung injury (ALI) is a life-threatening inflammatory disease of the respiratory system, characterised by high mortality rates and lack of effective clinical interventions. Emerging evidence suggests that traditional Chinese medicine (TCM) formulations may offer therapeutic benefits in managing inflammatory respiratory diseases. Jinbei decoction (JBD), a 12-herb TCM preparation currently used for pulmonary fibrosis, has shown preliminary therapeutic potential in ALI; however, mechanistic studies remain limited. This study systematically evaluated JBD's therapeutic efficacy and elucidated its molecular mechanisms in LPS-induced ALI. Survival analysis demonstrated that JBD significantly improved survival rates in a concentration-dependent manner, while histopathological evaluation revealed a marked reduction in pulmonary tissue damage. These effects were further supported by significant decreases in circulating levels of major pro-inflammatory cytokines, such as TNF-α, IL-6 and IL-1β. Network pharmacology analysis identified 111 molecular targets associated with ALI pathogenesis influenced by JBD components, highlighting the regulatory effect on inflammatory signalling pathways in macrophages as the key intervening mechanism. Specifically, JBD suppressed LPS-induced inflammatory responses by inhibiting ERK phosphorylation and blocking IKKα/β activation, thereby preventing NF-κB-dependent cytokine production in macrophages. Notably, astrapterocarpan was identified as the pr...