Caloranaerobacter longqiensis sp. nov., a novel thermophilic member of the family Thermohalobacteraceae with the ability of ferric iron reduction from deep-sea hydrothermal sulphide

作者：Guangyu Li, Jingwen Cheng, Xiupian Liu, Xiaoteng Fu, Yingbao Gai, Zhisheng Zhu, Jianning Wang, Xiaobo Zhang, Xiang Zeng, Zongze Shao · 发表于：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · 年份：2025 · DOI：10.1099/ijsem.0.006962 · 被引用次数：1 · 研究领域：Genomics and Phylogenetic Studies、Microbial Fuel Cells and Bioremediation、Microbial Community Ecology and Physiology

A thermophilic, strictly anaerobic, Gram-stain-negative and ferric iron-reducing bacterium, designated as DY30410 T , was isolated from a hydrothermal sulphide sample collected from the Southwest Indian Ocean Ridge during the cruise DY30 of R/V Da Yang Yi Hao . Cells of strain DY30410 T were rod-shaped with rounded ends and motile with one or more laterally inserted flagellum (flagella). Strain DY30410 T grew optimally at pH 6.5–7.0, 50 °C, with salinity (sea salts) of 35–45 g l −1 . Ferric oxyhydroxide, ferric chloride, thiosulphate and elemental sulphur can be used as electron acceptors. Glucose and starch are used for growth. Elemental sulphur or yeast extract is not necessary. The principal fatty acids of strain DY30410 T were iso-C 15:0 , C 16:0 , 3OH-iso-C 14:0 and anteiso-C 15:0 . No respiratory quinone was detected. Phylogenetically, strain DY30410 T branched within the family Thermohalobacteraceae with Caloranaerobacter ferrireducens DY22619 T being its closest phylogenetic relative (98.4% similarity), followed by the type species Caloranaerobacter azorensis MV1087 T (97.2%). Strain DY30410 T has a draft genome size of 2.57 Mbp and has 2,852 predicted coding sequences. The genome DNA G+C content of strain DY30410 T was 29.2 mol%. The in silico DNA–DNA hybridization between strain DY30410 T and its closest relatives, C. ferrireducens DY22619 T or C. azorensis MV1087 T , was all 45.5%. The average nucleotide identity values between strain DY30410 T and C. ferrireducens...