Ultra-mild bisulfite outperforms existing methods for 5-methylcytosine detection with low input DNA

作者：Qing Dai, Tanner Baldwin, Ruitu Lyu, Bryan C. Daniels, Chang Ye, Chen Cao, Chenyou Zhu, Diwen Fan, Liane Lin, Yushuai Liu, Yiding Wang, Chuan He · 发表于：Nature Communications · 年份：2025 · DOI：10.1038/s41467-025-66033-y · 被引用次数：9 · 研究领域：Epigenetics and DNA Methylation、Genomics and Rare Diseases、Genomics and Chromatin Dynamics

We present Ultra-Mild Bisulfite Sequencing (UMBS-seq), a method for 5-methylcytosine (5mC) detection that minimizes DNA degradation and background noise. UMBS-seq outperforms conventional bisulfite and enzymatic methyl-sequencing (EM-seq) methods in library yield, complexity, and conversion efficiency when applied to low-input DNA samples. In particular, its effectiveness with low-input cell-free DNA (cfDNA) and hybridization-based target capture highlights its potential for clinical applications, including 5mC biomarker detection and early disease diagnosis.