Integrated Transcriptomic and Metabolomic Analysis Elucidates the Impact of Acute Ammonia Stress on Carbohydrate and Lipid Metabolic Pathways in Yellow Catfish ( Pelteobagrus fulvidraco )

作者：Xue Li, Shidong Wang, Muzi Zhang, Ming Li, Chao Chen · 发表于：Aquaculture Nutrition · 年份：2025 · DOI：10.1155/anu/5545977 · 被引用次数：4 · 研究领域：Aquaculture Nutrition and Growth、Aquaculture disease management and microbiota、Physiological and biochemical adaptations

Ammonia stress (AS) constitutes a significant environmental challenge that impedes aquaculture development. In this investigation, histomorphology assessments, physiological, and biochemical parameter analyses, and multiomics approaches were employed to elucidate the impact of acute AS on yellow catfish ( Pelteobagrus fulvidraco ). Findings indicated that serum ammonia concentrations exhibited a dose‐dependent increase, correlating with the intensity and duration of stress. As the primary detoxification organ, the liver facilitates ammonia clearance by upregulating genes involved in glutamine and ureagenesis (glutamine synthase [ gs ], carbamoyl‐phosphate synthase [ cps ], ornithine transcarbamylase [ otc ], argininosuccinate lyase [ asl ], argininosuccinate synthase [ ass ], arginase [ arg ]), thereby promoting glutamine and ureagenesis while consuming glutamate, argininosuccinic acid, aspartic acid, arginine, and adenosine triphosphate (ATP). Physiological and biochemical data revealed that AS significantly elevated serum glucose, liver triglyceride (TG), and total cholesterol (TC) levels. Histological examination demonstrated a marked reduction in liver glycogen stores alongside a progressive accumulation of lipid droplets proportional to stress severity, suggesting activation of liver glycogenolysis coupled with suppression of lipolysis. Integrative transcriptomic and metabolomic analyses indicated a reprograming of liver energy metabolism characterized by enhanced glycog...