Differentiating Creatine and Phosphocreatine In Vivo Using 3 T 1 H MR Spectroscopy

作者：Ralph E. Hurd, Meng Gu, Philip M. Adamson, Kenichi Okamura, Masafumi Shibata, Yoshikazu Ono, Moussa Haidar, R. Kirk Riemer, Frank L. Hanley, Daniel M. Spielman · 发表于：Magnetic Resonance in Medicine · 年份：2025 · DOI：10.1002/mrm.70171 · 被引用次数：2 · 研究领域：Advanced MRI Techniques and Applications、Metabolomics and Mass Spectrometry Studies、Lanthanide and Transition Metal Complexes

PURPOSE: Validation and improvements to the proton MR spectroscopic measurement of creatine and phosphocreatine in vivo at 3 T. METHODS: Spectral simulations were used to evaluate and explore the mitigation of LCModel fitting bias due to lineshape, signal-to-noise (SNR) and non-metabolite backgrounds with a focus on phosphocreatine and the spline regularization settings in the fitting model. RESULTS: Although phosphocreatine and creatine are visually unresolved, excellent fitting estimates are achieved in the absence of non-metabolite background using default settings. However, in the presence of non-metabolite background, bias is observed and depends on lineshape, SNR and differences in the non-metabolite background. A more flexible setting for the LCModel spline largely mitigates this bias, allowing for the confidence to use proton MRS for PCr dynamic studies. CONCLUSION: Without prior knowledge of the non-metabolite signal, default settings of LCModel can introduce significant bias. Modified setup for LCModel improves quantitation of phosphocreatine and reduces bias in the overall set of metabolites.