Rooting for sustainability: Pantoea ananatis D1-28 volatiles boost tomato growth and rhizosphere microbial diversity

作者：Nan Zeng, Haiyang Gong, Jiahe Pang, Dandan Wang, Ruoyan Ran, Chunji Li, Die Zhao, Xinyue Bi, Zhiyong Zhang, Faryal Babar Baloch, Ning Zhang, Bingxue Li · 发表于：Chemical and Biological Technologies in Agriculture · 年份：2025 · DOI：10.1186/s40538-025-00882-z · 被引用次数：1 · 研究领域：Plant-Microbe Interactions and Immunity、Plant Molecular Biology Research、Plant Pathogenic Bacteria Studies

Tomato (Solanum lycopersicum), a vital global crop rich in bioactive compounds, faces sustainability issues due to agrochemical overuse, necessitating eco-friendly biofertilizers like plant growth-promoting rhizobacteria (PGPR) for sustainable agriculture. This study aimed to identify superior PGPR strains with growth-enhancing volatile organic compounds (VOCs), characterize their VOC profiles, determine optimal VOC doses for growth promotion, and investigate their effects on root architecture, rhizosphere microbiome, and plant transcriptomics to elucidate mechanisms. Thirteen PGPR strains were screened for VOC-mediated tomato growth promotion. Key strains' VOCs were profiled using GC–MS. Dose-response assays tested core VOCs on growth and root architecture. Rhizosphere microbiome functional compartmentalization was analyzed, and transcriptomic profiling (KEGG pathway enrichment) of VOC-treated plants was performed. Three superior isolates (Pantoea ananatis D1-28, Burkholderia sp. D4-24, Burkholderia territorii D4-36) were identified, with D1-28 notably enhancing lateral roots and shoot biomass. GC–MS revealed strain-specific VOC profiles (31–37 compounds) sharing three core components: dimethyl disulfide (D), 2-nonanone (N), benzothiazole (B). Optimal doses profoundly remodeled root architecture and maximized growth: D (10⁻3 mmol/L), N (1 mmol/L), B (10⁻2 mmol/L). VOCs drove rhizosphere functional compartmentalization, enriching specific taxa. Transcriptomics identified 132 ...