Effects of polysaccharides from Stropharia rugosoannulata on cyclophosphamide‐induced immunosuppression and hepatic injury in mice

作者：Li Wang, Yupeng Yang, Xuelian Tang, Chunnan Wen, Zhiqiang Zhang, Xia Ma, Bingji Ma · 发表于：Journal of the Science of Food and Agriculture · 年份：2025 · DOI：10.1002/jsfa.70295 · 被引用次数：7 · 研究领域：Polysaccharides and Plant Cell Walls、Seaweed-derived Bioactive Compounds、Liver physiology and pathology

BACKGROUND: The immune system is essential for human health. Traditional chemical immunomodulators often have toxic side effects; for example, long-term use of cyclophosphamide (CTX) can lead to severe adverse consequences. Stropharia rugosoannulata, a flavorful edible mushroom native to China, exhibits antioxidant, immunomodulatory, and antibacterial activity, with its polysaccharides being the key active components responsible for its immunomodulatory effects. RESULTS: Polysaccharides were extracted from S. rugosoannulata using a three-phase method to investigate their immunomodulatory activity and hepatoprotective effects in cyclophosphamide-induced mice. In vivo experiments showed that these polysaccharides significantly increased body weight, spleen index, and phagocytic index, regulated the CD4+/CD8+ ratio, and promoted secretion of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), immunoglobulin A (IgA), and immunoglobulin G (IgG). The mechanism involved activation of the TLR4/MyD88/NF-κB signaling pathway, effectively enhancing immune function. Regarding hepatic protection, S. rugosoannulata polysaccharides (SRP) increased serum malondialdehyde (MDA) and decreased superoxide dismutase (SOD), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels in immunosuppressed mice, reversing CTX-induced liver injury. Histopathological analysis confirmed that SRP ameliorated CTX-induced pathological changes, including single-cell ...