Isolation and in vitro evaluation of bacteriophages against Pseudomonas aeruginosa isolated from burn wounds of human patients and hospital environment

作者：Rabia Kanwar, Muhammad Aamir Aslam, Sajjad Ur Rahman, Muhammad Saqib · 发表于：Journal of Global Antimicrobial Resistance · 年份：2025 · DOI：10.1016/j.jgar.2025.10.012 · 被引用次数：2 · 研究领域：Bacteriophages and microbial interactions、Bacterial biofilms and quorum sensing、Bacillus and Francisella bacterial research

OBJECTIVE: Multidrug resistance Pseudomonas aeruginosa is a global public health issue, and bacteriophages are an effective alternative therapy over antibiotics to combat antibiotic resistance. This study was designed to isolate bacteriophages and determine their antimicrobial potential against P. aeruginosa isolated from burn wounds and the environment of the burn ward. METHODS: A total of 100 random swab samples were collected from the burn ward, including 50 from the burn wounds and 50 from the environment and proceeded for further investigations at the University of Agriculture Faisalabad. P. aeruginosa was confirmed by genomic detection of OprL gene. Bacteriophages were isolated from sewage water through the agar overlay method, and their lytic spectrum was determined. Genome sequencing and analysis was performed. Phage stability and synergism between phage and antibiotics were analysed. RESULTS: Overall, 50 samples were positive for P. aeruginosa, in which 35 of 50 were positive from wounds of patients with burns, and 15 of 50 were positive from the hospital environment. OprL-positive strains were selected for bacteriophage isolation. Two phages, PhPa-6 and PhPa-4, with lytic spectra of 88 % and 60 %, respectively, were selected. The genome sizes of PhPa-6 and PhPa-4 were 186.978 kb and 73.3 kb, respectively. A cocktail of these phages has shown an active in vitro synergistic effect with ciprofloxacin (0.125 µg/mL). CONCLUSIONS: A cocktail of PhPa-6 and PhPa-4 in combin...