Decadal manure substitution reshapes microbial communities to drive plant and microbial carbon accumulation in soil carbon fractions

作者：Zhengwu Wu, Yanyan Zhang, Yuchuan Fan, Changquan Wang, Yiran Li, Zed Rengel, Jianbo Shen, Edith Le Cadre, Joann K. Whalen, Xiaomei Tang · 发表于：Geoderma · 年份：2025 · DOI：10.1016/j.geoderma.2025.117581 · 被引用次数：9 · 研究领域：Soil Carbon and Nitrogen Dynamics、Microbial Community Ecology and Physiology、Clay minerals and soil interactions

The transformation of organic residues into particulate organic carbon (POC) and mineral-associated organic carbon (MAOC) governs soil organic carbon (SOC) storage and stability. However, how long-term manure use steers plant- and microbe-derived SOC fractions in paddy soils remains unclear. The SOC in paddy soil was evaluated by measuring amino sugars, lignin and enzyme activities from a 12-yr field trial (rice–wheat rotation) with no fertilizer, 100% mineral N fertilizer, and pig manure (50% and 100% replacement of N fertilizer). Compared to mineral N, the 100% pig manure treatment increased SOC by 26%, mainly through enhanced lignin input and microbial necromass accumulation, leading to significantly higher POC and MAOC levels. Manure application, compared to mineral N, promoted rice growth, which increased total organic C input and nutrient availability, thereby selectively favoring r -strategist bacteria, as reflected by a lower vanillyl acid-to-aldehyde ratio, a higher ratio of cellulose- to lignin-degrading enzyme activity, and greater lignin and bacterial necromass production. In contrast, the combined NPK and pig manure treatment increased the proportion of MAOC in total SOC by 7% compared to the 100% manure treatment, yet this came with enhanced lignin degradation, a lower bacterial phospholipid fatty acid (PLFA)/fungal PLFA ratio, and greater abundance of K -strategist fungi, resulting in less POC accumulation. These findings demonstrate that substituting mineral N...