Ceramic-Inspired Moldable Osteogenic Paste For Alveolar Bone Regeneration

作者：Rui Xu · 发表于：International Dental Journal · 年份：2025 · DOI：10.1016/j.identj.2025.105904 · 研究领域：Bone Tissue Engineering Materials、Periodontal Regeneration and Treatments、Dental Implant Techniques and Outcomes

Aim or purpose Current alveolar bone defect treatments rely on coagulation-dependent stabilization, limiting efficacy in patients with clotting disorders. This study aimed to develop a ceramic-inspired osteogenic (CIO) hydrogel for moldable, rapid-curing, and coagulation-independent bone regeneration. Materials and methods CIO hydrogel composed of gelatin methacrylate (GelMA), nanoclay (Laponite), and synthetic apatite was tested in New Zealand albino rabbits (male, 2.5-3 kg) and cynomolgus monkeys (male, 5 kg) with critical-sized cranial/alveolar defects. Ethics approvals: JGLI-20230710 (rabbits) and PL21-0613-1 (monkeys). Hydrogel rheology, mechanical strength, cytocompatibility, and osteogenic differentiation were assessed via SEM, FTIR, rheometry, and CCK-8/ALP assays. Bone regeneration was evaluated using micro-CT, histology (H&E, Masson’s trichrome), and biochemical analysis over 6 months. Results CIO hydrogel exhibited shear-thinning behavior (15 s UV curing), enhanced compressive strength (1.5-fold vs. GelMA-L, p<0.001), and promoted MSC proliferation/osteogenesis (ALP activity: 3.2-fold vs. control, p<0.001). In rabbits, CIO achieved 82% BV/TV vs. 28% in controls (p<0.01). Monkey alveolar defects showed regenerated bone with comparable BMD (1.12±0.15 g/cm³) and trabecular structure (Tb.Th: 0.21±0.03 mm) to autologous bone (p>0.05). Conclusions The CIO hydrogel enables moldable, rapid stabilization of bone grafts, overcoming coagulation limitations and restoring compl...