Neural network tissue and myocytes co-culture system reveals effects of different myocytes on postnatal development of spinal cord neural network

作者：Shang‐Bin Yang, Haiyang Yu, Jing Xu, Chuangran Wu, Liang Chen, Miao Tian, Ying Ding, Yue Yang, Zhen Chen, Mingyu Lv, Jiawei Sun, Yujian Lin, Kang Zhang, Xiangyu Liu, Yinan Guo, Ling Zhang, Jiafeng Fang, Bi‐Qin Lai · 发表于：Cellular and Molecular Life Sciences · 年份：2025 · DOI：10.1007/s00018-025-05872-w · 被引用次数：2 · 研究领域：Neurogenesis and neuroplasticity mechanisms、Pluripotent Stem Cells Research、Nerve injury and regeneration

The spinal cord serves as a vital bridge connecting the brain and all the organs of the body, and its development and function are affected by other organs [ 1 , 2 , 3 ]. The interaction between the spinal cord neural network and myocytes is essential for the development and maturation of the nervous system, and is closely related to somatic movement and visceral functions [ 4 , 5 ]. Skeletal muscle cells (SkMCs) are innervated by spinal cord motor neurons [ 2 ], and vascular smooth muscle cells in central nervous tissue and corpus cavernosum smooth muscle cells (CC-SmMCs) are also innervated by spinal cord neuron [ 6 , 7 ]. There have been studies on the interaction between central nervous system (CNS) organoids and SkMCs, which simulate the impact of skeletal muscle on the development and functional formation of the CNS during the early stages of development [ 8 , 9 ]. However, little is known about the mechanism by which SkMCs and CC-SmMCs, influence the postnatal development of the spinal cord neural network [ 10 ]. In contrast to the embryonic stage, postnatal development of the central nervous system relies more heavily on the refinement of synaptic activity and the feedback regulation of neural function by muscle activity [ 11 ]. Given that the maturation of neural circuits and neuromuscular junctions predominantly occurs during the postnatal period, investigating normal postnatal neuromuscular development is essential for understanding pediatric neuromuscular disorder...