An innovative strategy for overcoming ultra-high ammonia nitrogen inhibition on anaerobic methanogenesis via stepwise domestication

作者：Heng Wu, Taili Dong, Anjie Li, Jing Chen, Huaiwen Zhang, Hongyi Lv, Caiyun Yang, Xiaohui Guo, Xuanmin Yang, Ling Qiu, Changlin Miao, Yiqing Yao · 发表于：Microbiome · 年份：2025 · DOI：10.1186/s40168-025-02255-y · 被引用次数：5 · 研究领域：Anaerobic Digestion and Biogas Production、Microbial Fuel Cells and Bioremediation、Wastewater Treatment and Nitrogen Removal

The world is facing both an increasingly severe energy crisis and a growing problem of agricultural pollution. The utilization of agricultural waste by anaerobic digestion (AD), has received increasing attention. AD using representative waste cow dung results in total ammonia nitrogen (TAN) accumulation and inhibition of methanogens resulting in reduced CH 4 production. However, there is a lack of highly efficient in-situ biological domestication strategies to enhance the TAN tolerance of methanogens in AD systems. In this study, an incremental approach to gradually increasing the TAN concentration has been used for overcoming the problem. The results showed that at an ultra-high concentration of 6124.09 mg/L TAN, a 48-day domesticated AD system functioned stably and the cumulative CH 4 production reached 72.81 mL/g volatile solids, whereas the undomesticated AD system failed to produce CH 4 . After domestication, the lactate dehydrogenase concentration decreased to 96.44 ng/L and the adenosine triphosphate concentration increased to 48.77 nmol/L, confirming that microbial activity improved. Hydrolytic and acidogenic bacteria were enriched, with Methanosarcina (79.73%) dominating the domesticated AD system, primarily Methanosarcina mazei . Metagenomic analysis showed that with two-component system enrichment, the key inhibited steps from glycerate-1,3P2 to pyruvate (2.498‰), and from acetyl-CoA and acetyl phosphate to acetic acid (1.141‰ and 0.798‰), as well as vital methanog...